The core function of the anti trypsin assay kit is to quantitatively detect the concentration of α 1-antitrypsin (α 1-AT/AAT) in biological samples. Its applications cover multiple fields such as clinical diagnosis, disease monitoring, scientific research analysis, and efficacy evaluation. The specific functions are as follows:
1. Clinical diagnosis: auxiliary identification and evaluation of diseases
Liver disease diagnosis
α 1-antitrypsin is mainly synthesized by the liver, and its serum concentration is an important indicator for evaluating liver synthesis function.
.Application scenario: The level of α 1-AT in the serum of patients with liver diseases such as cirrhosis and hepatitis may decrease, and the reagent kit can assist in determining the degree of liver damage.
.Diagnosis of lung diseases
Alpha 1-aT deficiency (such as alpha 1-antitrypsin deficiency induced emphysema) can lead to the destruction of lung tissue by proteases, causing chronic obstructive pulmonary disease (COPD), cystic fibrosis, and other diseases.
.Application scenario: By detecting the concentration of α 1-AT in serum or bronchoalveolar lavage fluid, it can assist in the diagnosis of α 1-AT deficiency and guide personalized treatment (such as supplementing α 1-AT preparations).
.Diagnosis of intestinal diseases
Loss of intestinal proteins (such as Crohn's disease and necrotizing enterocolitis) can lead to an increase in the concentration of α 1-AT in feces, as it has resistance to digestive enzyme degradation.
.Application scenario: Detecting the concentration of α 1-AT in feces, assisting in the diagnosis of intestinal protein loss diseases, and evaluating intestinal mucosal permeability.
.Tumor biomarker detection
The concentration of α 1-AT in gastric juice of patients with gastric cancer, peptic ulcer and other diseases may be abnormally elevated.
.Research case: Using an ELISA kit to detect the concentration of α 1-AT in gastric juice, it was found that the concentration in the gastric cancer group was significantly higher than that in the peptic ulcer group and gastritis group. ROC curve analysis showed that its diagnostic sensitivity reached 97.3%, providing a new method for gastric cancer screening.
.II. Disease Monitoring: Dynamic Tracking of Disease Progression
Chronic Disease Management
Patients with chronic obstructive pulmonary disease (COPD) need to regularly monitor their levels of α 1-AT to evaluate disease progression and treatment effectiveness.
.Application scenario: By tracking serum α 1-AT concentration for a long time, adjust the treatment plan (such as whether to supplement α 1-AT preparations).
.Evaluation of inflammatory response
α 1-AT is an acute phase reactive protein, and its serum concentration increases during inflammatory response.
.Application scenario: Detecting serum α 1-AT concentration to assist in determining the severity of inflammatory diseases such as infection and trauma.
.III. Scientific Analysis: Exploring Disease Mechanisms and Biomarkers
Inflammatory Response Research
α 1-AT protects tissues from inflammatory damage by inhibiting proteases such as neutrophil elastase.
.Research scenario: In animal models or cell experiments, detecting changes in α 1-AT concentration and studying its regulatory role in inflammatory response.
.Research on the mechanism of pulmonary fibrosis
Lack of α 1-AT can lead to excessive repair of lung tissue, causing pulmonary fibrosis.
. Research scenario: By detecting the concentration of α 1-AT in lung tissue or bronchoalveolar lavage fluid, explore its relationship with the pathogenesis of pulmonary fibrosis.Research on tumor development
α 1-AT may be involved in regulating the tumor microenvironment, affecting tumor cell proliferation and migration.
.Research scenario: Detecting the concentration of α 1-AT in tumor tissue or serum and studying its potential as a tumor biomarker.
.Fourth, efficacy evaluation: guiding treatment and prognosis judgment
Monitoring of alpha 1-AT replacement therapy
Patients with alpha 1-AT deficiency need to regularly supplement alpha 1-AT preparations, and serum concentration testing can evaluate treatment efficacy.
.Application scenario: By using a reagent kit to detect the levels of α 1-AT before and after treatment, adjust the dosage and frequency of medication.
.Drug development and evaluation
In drug clinical trials, detecting the concentration of α 1-AT can evaluate the effect of drugs on liver synthesis function or inflammatory response.
.Application scenario: For example, studying the effect of novel anti-inflammatory drugs on the expression of α 1-AT, verifying their efficacy and safety.
.source:http://en.zjikon.com/news1081626.html